Qiagen Buffer Recipes 2021 -

Washes the column while keeping DNA bound, removes salts. Components: Tris-HCl, pH 7.5 Preparation: Mix Tris-HCl (pH 7.5) with Ethanol. Bring to 1 Liter with dH2Od cap H sub 2 cap O . Store at RT . Buffer EB (Elution Buffer) Purpose: Elutes pure DNA from the column. Components: Tris-HCl, pH 8.5 Alternative: Sterile dH2Od cap H sub 2 cap O or TE buffer can be used instead. 3. Gel Extraction Buffer (Buffer QG) Purpose: Dissolves agarose gel slices and binds DNA. Components: Guanidine Thiocyanate (GuSCN) Tris-HCl, pH 6.6 Preparation: Dissolve dH2Od cap H sub 2 cap O Tris-HCl (pH 6.6). Mix well. Store at RT . 4. Anion Exchange Buffers (Midi/Maxi Kits) Buffer QBT (Equilibration Buffer) Components: MOPS, pH 7.0, Isopropanol, Triton X-100. Buffer QC (Wash Buffer) Components: MOPS, pH 7.0, Isopropanol. Buffer QF (Elution Buffer) Qiagen Plasmid Prep - Buffer Composition

(Note: This differs from P3 used in Maxi preps) This is often replaced with

Here is a practical guide to the recipes for common Qiagen buffers, along with the chemistry that makes them work. qiagen buffer recipes

Genomic DNA prep (blood, tissue) Function: Proteinase K activation + chaotropic lysis.

Ensures DNA binds to the silica column in high-salt/chaotropic conditions. Components: Guanidine-HCl Isopropanol Preparation: Dissolve Guanidine-HCl in dH2Od cap H sub 2 cap O Isopropanol. Bring to 1 L. Store at RT . Buffer PE (Wash Buffer) Washes the column while keeping DNA bound, removes salts

SDS solution. . If SDS precipitates, warm to 37°C before use . Buffer N3 (Neutralization Buffer)

Below is a for preparing equivalent buffers. Always use nuclease-free water for RNA work. Store at RT

While these recipes are excellent for saving money or troubleshooting, they are approximations of the commercial product.

and Buffer PB contain NaOH or high guanidine-HCl, which can cause irritation. Use appropriate PPE.