Molarity Graphpad !exclusive! -
Once you have your experimental results (e.g., absorbance from an ELISA or radioactivity from a binding assay), you can use the GraphPad Prism desktop software to turn those values back into molarity units. Step 1: Interpolating from a Standard Curve
Prism’s built-in dose-response models expect .
The most common workflow for determining unknown molarities is to create a standard curve: molarity graphpad
Determine exactly how many grams of a compound you need to weigh out to reach a target molarity.
Even with the best software, the accuracy of your molarity calculations depends on your inputs: Molarity Calculator - GraphPad Once you have your experimental results (e
To plot a standard curve, follow these additional steps:
To create a molarity graph in GraphPad, follow these steps: Even with the best software, the accuracy of
: The calculator supports units from femtomolar to molar , and Prism allows you to easily transform these values (such as converting to log scale) for non-linear regression analysis.
| Parameter | Report as | |-----------|------------| | EC50 | 1.2e-7 M (95% CI: 8.5e-8 to 1.7e-7) | | LogEC50 | -6.92 ± 0.08 | | Hill slope | 1.2 ± 0.15 | | Top | 98.3% ± 2.1 | | Bottom | 4.2% ± 1.3 |
| X (M) | Drug A - Rep1 | Drug A - Rep2 | Drug B - Rep1 | Drug B - Rep2 | |-------|---------------|---------------|---------------|---------------|